Isolation and molecular characterization of feline panleukopenia viruses from Korean cats

Feline panleukopenia virus (FPV) causes fatal leukopenia and severe hemorrhagic diarrhea in cats. Although FPV isolates have been reported worldwide from several animals, the biological genetic features of South Korean FPVs remain unclear. We characterized molecularly isolates. Crandell-Rees feline kidney (CRFK) cells were used to isolate 60 organ homogenates. The confirmed be via analyses cytopathic effects, immunofluorescence studies, electron microscopy, polymerase chain reaction. Viral full VP2 sequences. Eight propagated CRFK FPVs. All yielded viral titers ranging 104.5 106.0 TCID50/mL 5 days after inoculation into exhibited hemagglutination 27 212 (using pig erythrocytes). grew well cat such as Fcwf4 cells. most similar KS42 strain isolated a 2008. will serve useful antigens future sero-epidemiological studies aid development diagnostic tools.